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KMID : 0895820200300020067
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Journal of Oriental Rehabilitation Medicine
2020 Volume.30 No. 2 p.67 ~ p.75
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Antioxidant Activity and Standardization of Extraction Solvents of SJ004
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Lee Dae-Yeon
Jo Ju-Hwi
Kim Wan-Su
Lee Ho-Sung
Lee Young-Woo
Park Sang-In
An Keon-Sang
Lee In-Hee
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Abstract
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Objectives: SJ004 is a natural herbal medicine that contains Acyranthes japonica Nakai and Eucommia ulmoides Oliver traditionally used for joint and spinal diseases. This study aimed to establish an efficient method of extracting SJ004 to standardize using the yield, high-performance liquid chromatography (HPLC), and antioxidant assay.
Methods: SJ004 was extracted with distilled water, 70% and 100% of ethyl alcohol (EtOH). The method validation of 20-hydroxyecdysone and pinoresinol diglucoside was determined by HPLC-photo diode array and the content of SJ004 was calculated. The antioxidant activity of each extract was compared and measured using total flavonoids, total phenolic compounds, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and ferric reducing antioxidant power according to the standard protocol.
Results: The yield was highest in pure water extract and lowest in 100% EtOH. But, the content of marker compounds indicating 20-hydroxyecdysone and pinoresinol diglucoside was highest in 100% EtOH extract. In the physiological activity measurement using antioxidant activity, 100% ethanol extract was highest. The limit of detection indicating 20-hydroxyecdysone and pinoresinol diglucoside were analyzed 0.33 μg/mL, 0.1616 μg/mL, and the limit of quantification were analyzed 1.01 μg/mL and 0.49 μg/mL respectively.
Conclusions: The experimental results showed that the extraction conditions have a significant effect on content of marker compounds and antioxidant activity. As a result of method validation, SJ004 was standardized by 20-hydroxyecdysone and pinoresinol diglucoside.
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KEYWORD
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SJ004, Antioxidants, High pressure liquid chromatography, Ecdysterone
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